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Article Title: Genes Specific for the Biosynthesis of Clavam Metabolites Antipodal to Clavulanic Acid Are Clustered with the Gene for Clavaminate Synthase 1 in Streptomyces clavuligerus

doi:

Figure Lengend Snippet: Bacterial strains and plasmids used in this study

Article Snippet: Production cultures were grown under conditions similar to those used for seed cultures. table ft1 table-wrap mode="anchored" t5 TABLE 1 caption a7 Strain or plasmid Description Reference or source Strains Bacillus sp. ATCC 27860 Indicator strain; sensitive to alanylclavam on minimal medium 26 E. coli ER1447 ara-14 F − dam-13 ::Tn 9 (Cm r ) dcm6 fhuA31 galK2 galT22 hisG4 hsdR2 (r k − m k + ) lacY1 leuB6 mcrA mcrB1 mtl-1 rpsL136 (Str r ) supE44 thi-1 tsx-78 xyl-5 J. McCormick, Harvard University, Cambridge, Mass.

Techniques: Plasmid Preparation, Northern Blot, Clone Assay, Isolation, Modification

Journal: Brazilian Journal of Microbiology

Article Title: Genome of Bacillus sp. strain QHF158 provides insights into its parasporal inclusions encoded by the S-layer gene

doi: 10.1007/s42770-019-00205-z

Figure Lengend Snippet: Phylogenetic analyses of eight genomes of Bacillus sp. strain QHF158 and representative related Bacillus species by OAT. The genomic sequences of Bacillus cereus ATCC 14579 (NC004722), Bacillus mycoides strain ATCC 6462 (CP009692), Bacillus thuringiensis serovar kurstaki strain HD-1 (CP004870), Bacillus thuringiensis serovar israelensis strain 4Q7 (JEOC01000001), Bacillus toyonensis BCT-7112 (NC022781), Bacillus wiedmannii strain FSL W8-0169 (LOBC01000001), and Bacillus subtilis strain 168 (NC000964) downloaded from NCBI for phylogenetic analysis

Article Snippet: The phylogenetic tree was constructed using 16S rDNA sequences retrieved from GenBank, which indicated Bacillus sp. strain QHF158 is most closely related to B. mycoides strain ATCC 6462 instead of B. thuringiensis (SI Fig. 1 ).

Techniques: Genomic Sequencing

Journal: Brazilian Journal of Microbiology

Article Title: Genome of Bacillus sp. strain QHF158 provides insights into its parasporal inclusions encoded by the S-layer gene

doi: 10.1007/s42770-019-00205-z

Figure Lengend Snippet: Micrograph and SDS-PAGE profile of parasporal crystalline inclusions of strain QHF158 during the sporulation (A). Micrograph of a sporulated cell under × 100 oil immersion objective optical microscope (B). Scanning electron micrographs showing spores (SP) and parasporal crystals (PCI) of strain QHF158 (C). SDS-PAGE profile of parasporal inclusions from strain QHF158, PM is the protein molecular weight marker

Article Snippet: The phylogenetic tree was constructed using 16S rDNA sequences retrieved from GenBank, which indicated Bacillus sp. strain QHF158 is most closely related to B. mycoides strain ATCC 6462 instead of B. thuringiensis (SI Fig. 1 ).

Techniques: SDS Page, Microscopy, Molecular Weight, Marker

Journal: Brazilian Journal of Microbiology

Article Title: Genome of Bacillus sp. strain QHF158 provides insights into its parasporal inclusions encoded by the S-layer gene

doi: 10.1007/s42770-019-00205-z

Figure Lengend Snippet: MS-MASCOT search results for the TOF data from 90 kDa protein of strain QHF158

Article Snippet: The phylogenetic tree was constructed using 16S rDNA sequences retrieved from GenBank, which indicated Bacillus sp. strain QHF158 is most closely related to B. mycoides strain ATCC 6462 instead of B. thuringiensis (SI Fig. 1 ).

Techniques: